We rely on soil to support the crops on which we depend. Less obviously we also rely on soil for a host of 'free services' from which we benefit. For example, soil buffers the hydrological system greatly reducing the risk of flooding after heavy rain; soil contains very large quantities of carbon, which would otherwise be released into the atmosphere where it would contribute to climate...
Intercellular variability can be both a source of information and a confounding factor in understanding signal propagation in biological networks. It is crucial to properly sample the population, which demands high-throughput methods. In turn, these require not only automated microscopy, but also automated analysis. Therefore, we developed a set of algorithms to address that challenge. Using a...
Mitochondria form contacts to endosomes and endoplasmic reticulum, but little is known about the dynamics of such membrane contact sites (MCSs). Mitochondria receive cholesterol from late endosomes and lysosomes (LE/LYSs) and the plasma membrane (PM) for production of oxysterols and steroid hormones in a process depending on the endo-lysosomal sterol transfer protein Niemann Pick C2 (NPC2). In...
