11-12 November 2021
Digital (Zoom)
Europe/Berlin timezone

Evolution of ipaH family proteins in human and non-human hosts Escherichia

12 Nov 2021, 14:00
Digital (Zoom)

Digital (Zoom)


Ms Natalia O. Dranenko (Kharkevich Institute for Information Transmission Problems, Moscow, Russia)


Shigella and enteroinvasive Escherichia coli, human-restricted pathogens, can enter epithelial cells of the colon, multiplicate within them, and move between adjacent cells using the type 3 secretion system (T3SS) encoded by the pINV virulence plasmid. A large family of T3SS effectors, E3 ubiquitin-ligases encoded by the ipaH genes, plays a key role in the Shigella pathogenicity via modulation of the cellular ubiquitination leading to the degradation of host proteins. Nevertheless, ipaH gene repertoire in the genus and its impact on strain virulence is still unclear. Recently, the presence of T3SS and ipaH genes in Escherichia marmotae, a potential marmot pathogen, was declared. We performed comparative genomic analysis of the ipaH genes in Shigella and enteroinvasive Escherichia from human and non-human hosts. Shigella ipaH genes as well as their regulatory elements are highly conserved but less than half of Shigella genomes held a complete set of ipaH genes; gene losses and duplications are not consistent to species tree and nomenclature. In contrast to Shigella, non-human-host IpaH proteins reveal two different types of NEL
C-terminal domain and a diverse set of subtract-binding domains; only ipaH9.8 gene was found both in human-host and non-human-host Escherichia. These results provide a framework for understanding of host-pathogens interactions and the role of effectors’ composition.

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