Whole organ quantitative biology with light sheet microscopy to uncover the impact of sterile and infectious inflammation on tissue integrity
Organs are delicate 3-D structures with high degrees of functional organization. These structures are affected on the microscopical but also meso- and macroscopical level by pathological conditions. We have been using light sheet fluorescence microscopy (LSFM) to investigate the fine details of organs in unprecedented detail with a focus on blood vessels systems and infiltrating immune cells. In this talk I will briefly introduce the principal method and then demonstrate on several examples how inflammation massively affects a delicately structured environment.